Coding

Part:BBa_K2557047:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


Bxb1-RDF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1566
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1566
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 774
    Illegal BglII site found at 1572
    Illegal BamHI site found at 215
    Illegal BamHI site found at 531
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 1530
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1566
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1566
    Illegal NgoMIV site found at 516
    Illegal NgoMIV site found at 2049
    Illegal NgoMIV site found at 2232
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 236


Design Notes

We have optimized the codons.


Source

We found the sequence of Bxb1-RDF from the literature and synthesized this sequence

References